An Outbreak of Vibriosis in Wild Ayu (Plecoglossus altivelis) and Characteristics of the Isolates
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چکیده
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Cloning and characterization of metallothionein gene in ayu Plecoglossus altivelis.
Metallothionein (MT) has been used widely as a potential molecular marker to detect the deleterious effects of heavy metals in aquatic ecosystem. Here we exposed ayu, Plecoglossus altivelis, to zinc (Zn) and tested the distribution as well as the induction of MT in various tissues such as liver, kidney, intestine and stomach. MT induction was significant in liver tissue, followed by kidney and ...
متن کاملPlasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan
In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other...
متن کاملStudies on Morphology and Cytochemistry in Blood Cells of Ayu Plecoglossus altivelis altivelis
Peripheral blood cells from ayu, Plecoglossus altivelis altivelis, were separated using a density gradient. Blood cells were then smeared using Shandon Cytospin and subjected to cytochemical staining. Blood cells were categorized based on morphological and cytochemical characteristics, and the density fractionation range and nucleus area/cell area ratio were observed. Lymphocytes are distinguis...
متن کاملCharacterization of P2X7R and Its Function in the Macrophages of ayu, Plecoglossus altivelis
P2X purinoceptor 7 (P2X7R), an ATP-gated ion channel, plays an important role during the innate immune response in mammals. However, relatively little is known about the role of P2X7R in the fish immune system. Here, we cloned a cDNA sequence encoding ayu (Plecoglossus altivelis) P2X7R (aP2X7R). The predicted protein was composed of 574 amino acid residues with a P2X family signature, two trans...
متن کاملProlactin and upstream migration of the amphidromous teleost, ayu Plecoglossus altivelis.
Changes in mRNA levels of prolactin (PRL) during the upstream migration were examined in fry of the amphidromous fish, ayu Plecoglossus altivelis. Quantification of mRNA has been done with real-time PCR and expressed as whole body or pituitary contents depending the body size of fry. PRL mRNA levels of ayu caught in seawater of the coastal area remained low during early spring. Prior to the sta...
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ژورنال
عنوان ژورنال: Journal of the Japan Veterinary Medical Association
سال: 1992
ISSN: 0446-6454,2186-0211
DOI: 10.12935/jvma1951.45.203